Dominant-Negative Proteins in Herpesviruses – From Assigning Gene Function to Intracellular Immunization

Dominant-Negative Proteins in Herpesviruses – From Assigning Gene Function to Intracellular Immunization

Investigating and assigning gene functions of herpesviruses is a process, which profits from consistent technical innovation. Cloning of bacterial artificial chromosomes encoding herpesvirus genomes permits nearly unlimited possibilities in the construction of genetically modified viruses. Targeted or randomized screening approaches allow rapid identification of essential viral proteins. Nevert...

Assigning function to yeast proteins by integration of technologies.

Interpreting genome sequences requires the functional analysis of thousands of predicted proteins, many of which are uncharacterized and without obvious homologs. To assess whether the roles of large sets of uncharacterized genes can be assigned by targeted application of a suite of technologies, we used four complementary protein-based methods to analyze a set of 100 uncharacterized but essent...

An Hsp27-related, dominant-negative-acting intracellular estradiol-binding protein.

New World primates (NWPs) exhibit a compensated form of resistance to gonadal steroid hormones. We demonstrated recently that estrogen resistance in NWP cells was associated with the overexpression of two proteins, a nonreceptor-related, dominant-negative-acting estrogen response element (ERE)-binding protein (ERE-BP) and an intracellular estradiol-binding protein (IEBP). Based on the N-termina...

Assigning gene function in biosynthetic pathways: camalexin and beyond.

Assigning Function Labels to Unparsed Text

In this paper, we propose a novel solution to the problem of assigning function labels to syntactic constituents. This task is a useful intermediate step between syntactic parsing and semantic role labelling. What distinguishes our proposal from other attempts in function or semantic role labelling is that we perform the learning of function labels at the same time as parsing. We reach state-of...